pcdna myc tcf 4 (Addgene inc)
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pcdna myc tcf 4
Pcdna Myc Tcf 4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+myc+tcf+4/pcDNA%2FMyc+TCF4+(Plasmid+%2316512)/pmc12935169-357-5-7
Average 92 stars, based on 20 article reviews
Pcdna Myc Tcf 4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna+myc+tcf+4/pcDNA%2FMyc+TCF4+(Plasmid+%2316512)/pmc12935169-357-5-7
Average 92 stars, based on 20 article reviews
pcdna myc tcf 4 - by Bioz Stars,
2026-10
92/100 stars
Images
1) Product Images from "4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein"
Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein
Journal: Chemmedchem
doi: 10.1002/cmdc.202500996
Figure Legend Snippet: Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).
Techniques Used: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot
Related Articles
Transfection:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. Co-Immunoprecipitation Assay:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. Immunoprecipitation:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. Western Blot:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. Plasmid Preparation:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. Incubation:Article Title: Novel N -(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. Article Snippet: Luciferase assays were performed as previously described.58,59 Briefly, HCT116 cells were transfected with Topflash reporter (M50 Super 8x TOPFlash, containing 8 repeats of TCF/LEF binding sites) or Fopflash reporter (M51 Super 8x FOPFlash, containing the mutated TCF/LEF binding sites) plasmids using the Dreamfect Gold Transfection Reagent (#DG80500, OZ Biosciences). Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein Article Snippet: Coelenterazine (#S053 Synchem) was diluted in PBS with Ca 2+ and Mg 2+ at a final concentration of 0,75 μg/mL. |